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Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nucl...


Gabriel, C.J.Walsh, RNolt, Barry L.[Postharvest root deterioration or vascular streaking of cassava clones (accessions and breeding lines) was evaluated with harvests at different ages (8 and 12 mo.), seasons (beginning and end of wet season), and locations (CIAT-Palmira, Carimagua, and Caribia in Colombia) to assess the effect of environmental factors on cassava genotypes. Vascular streaking was sensitive to any of these environmental factors. Genotype x location interaction was highly significant suggesting that final cv. selection should be carried out in each location. Genetic analysis carried out in one location suggested that inheritance of vascular streaking was, at least partly, controlled by additive factors and was quantitative rather than qualitative. Narrow sense heritability of 0.64 was obtained. Vascular streaking was highly and significantly correlated with root DM content in all the trials conducted during 7 yr. Since free recombination between resistance to vascular streaking and high root DM content may not be possible, the most acceptable balance between these 2 traits should be defined according to the use of product and edaphoclimatic condition of production area prior to selection program. (AS)]Postharvest root deterioration or vascular streaking of cassava clones (accessions and breeding lines) was evaluated with harvests at different ages (8 and 12 mo.), seasons (beginning and end of wet season), and locations (CIAT-Palmira, Carimagua, and Caribia in Colombia) to assess the effect of environmental factors on cassava genotypes. Vascular streaking was sensitive to any of these environmental factors. Genotype x location interaction was highly significant suggesting that final cv. selection should be carried out in each location. Genetic analysis carried out in one location suggested that inheritance of vascular streaking was, at least partly, controlled by additive factors and was quantitative rather than qualitative. Narrow sense heritability of 0.64 was obtained. Vascular streaking was highly and significantly correlated with root DM content in all the trials conducted during 7 yr. Since free recombination between resistance to vascular streaking and high root DM content may not be possible, the most acceptable balance between these 2 traits should be defined according to the use of product and edaphoclimatic condition of production area prior to selection program. (AS)

Project SB-2 was known as both Conservation and Use of Tropical Genetic Resources and, later, Assessing and Utilizing Agrobiodiversity through Biotechnology.


International Center for Tropical Agriculture[CIAT Biotechnology Research Unit: Project SB-2: Annual Reports 1985-2006]CIAT Biotechnology Research Unit: Project SB-2: Annual Reports 1985-2006

Fregene, Martin A.Ceballos, H.Roca, William M.Okogbenin, EmmanuelAkano, A.Mba, CChavarriaga Aguirre, PaulTohme, Joseph M.Ospina Patiño, Bernardo[Cassava biotechnology research at CIAT/Colombia]Cassava biotechnology research at CIAT/Colombia

Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, an...


Roca, William M.Nolt, Barry L.Mafla, G.Roa E, J.C.Reyes, R.[Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)]Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)

In vitro conservation strategies are reviewed, with special reference to in vitro active and basic gene banks; likewise, important aspects that should be considered for in vitro conservation, such as regeneration, viability, genetic stability, and information systematization, are discussed. The 2...


Roca, William M.Arias, D.I.Chavez, R.[Metodos de conservacion in vitro del germoplasma]Metodos de conservacion in vitro del germoplasma

Cassava is probably the most efficient producer of carbohydrate per unit land area under tropical conditions. The high productivity of cassava makes it an attractive source of renewable industrial raw material, provided ways are found to reduce production costs and solve constraints. Cassava has ...


Fregene, Martin A.Ceballos, H.Roca, William M.Okogbenin, EmmanuelAkano, A.Mba, ChikeluChavarriaga Aguirre, PaulTohme, Joseph M.Ospina Patiño, Bernardo[Cassava biotechnology research at CIAT/Colombia]Cassava biotechnology research at CIAT/Colombia

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