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Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nucl...
Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction ...
Cassava vegetative material is distributed by sending seedlings obtained from meristem cultures in test tubes. Although this material is accepted worldwide, losses occur due to seedling weakness and the delay to obtain adult seedlings in the field (normally between 3 and 4 yr). To overcome this p...
The overall and specific objectives of a collaborative research project between the International Board for Plant Genetic Resources (IBPGR) and CIAT are given. The overall objective is to assess and demonstrate the technical and logistic aspects of establishing and operating a pilot in vitro acti...
For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germplasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro ...
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