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Three species of wild African ruminants. impala (Aepyceros melampus), sable (Hippotragus equinus, and tsessebe (Damaliscus lunatus), were experimentally inoculated with in vitro culture-derived Cowdria ruminantium organisms, the tick-borne causative agent of heartwater in domestic ruminants, to d...


Peter, T.F.Anderson, E.C.Burridge, M.J.Perry, Brian D.Mahan, S.M.[Susceptibility and carrier status of impala, sable and tsessebe for Cowdria ruminantium infection (heartwater)]Susceptibility and carrier status of impala, sable and tsessebe for Cowdria ruminantium infection (heartwater)

Analysis of cattle major histocompatibility complex (MHC) (BoLA) class I gene expression using serological and biochemical methods has demonstrated a high level of polymorphism. However, analysis of class I cDNA sequences has failed to produce conclusive evidence concerning the number and nature ...


Ellis, S.A.Holmes, E.C.Staines, K.A.Smith, K.B.Stear, M.J.McKeever, Declan J.MacHugh, Niall D.Morrison, W. Ivan[Variation in the number of expressed MHC genes in different cattle class I haplotypes]Variation in the number of expressed MHC genes in different cattle class I haplotypes

Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index...


Nene, VishvanathLee, D.Quackenbush, J.Skilton, Robert A.Mwaura, S.Gardner, M.J.Bishop, Richard P.[AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum]AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum

Isometamidium chloride has remained a very important prophylactic and therapeutic drug against trypanosomosis in cattle since its introduction into the market in the 1950s with, unfortunately, a concomitant development of resistance in trypanosomosis endemic areas. Amplified Fragment Length Polym...


Delespaux, V.Geysen, D.Majiwa, P.A.O.Geerts, S.[Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense]Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense
Conrad, P.A.Ole-MoiYoi, O.K.Baldwin, C.L.Dolan, T.T.O'Callaghan, C.J.Njamunggeh, R.E.G.Grootenhuis, J.G.Stagg, D.A.Leitch, B.L.Young, A.S.[Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes]Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes
Pelle, Roger[The use of electrophoretic profile of RNA to differentiate Trypanosoma brucei from T. congolense]The use of electrophoretic profile of RNA to differentiate Trypanosoma brucei from T. congolense
Dube, D.K.Williams, R.O.Seal, G.Williams, S.C.[Detection and characterization of DNA polymerase from Trypanosoma brucei]Detection and characterization of DNA polymerase from Trypanosoma brucei

Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or se...


Sharma, K.Bhattacharjee, R.Sartie, A.M.Kumar, P.L.[An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction]An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction

Fifty-four accessions of Phaseolus vulgaris cultivated in Spain and representing a broad variability for this country, were studied together with 30 samples of wild forms of American origin. Two reference cultivars (from the Andes and Mesoamerica) plus two outgroups (P. coccineus and Vigna unguic...


Ocampo Nahar, César H.Martin, JPSánchez Yélamo, María DoloresOrtiz, J.M.Toro Chica, Orlando[Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers]Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers

We determined DNA fingerprints and pathotypes on international differentials for 151 isolates of the rice blast fungus Pyricularia grisea that were collected from 15 rice cultivars at a blast disease breeding nursery in Santa Rosa, Colombia. The pathogen population expressed an extraordinarily hi...


Levy, MCorrea Victoria, Fernando J.Zeigler, Robert S.Xu, SHamer, JE[Genetic diversity of the rice blast fungus in a disease nursery in Colombia]Genetic diversity of the rice blast fungus in a disease nursery in Colombia

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