In a cross-sectional study carried out in June 1994, 486 cattle from 50 randomly selected dairy farms in the vicinity of Kampala, Uganda, were examined for trypanosome infections. The trypanosome prevalence (either positive with the haematocrit centrifugation technique [HCT] and/or the mini-anion...
Clausen, P.H.
,
Schares, G.
,
Patzelt, R.J.
,
Poetzsch, C.J.
,
Scheer, A.
,
Kakaire, D.
,
Olila, D.
,
Peregrine, A.S.
,
McDermott, John J.
,
Mehlitz, D.
,
[PCR and DNA-probe hybridization to assess the efficacy of therapeutic and prophylactic isometamidium treatment in Trypanosoma spp. - infected dairy cattle in peri-urban Kampala, Uganda]
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PCR and DNA-probe hybridization to assess the efficacy of therapeutic and prophylactic isometamidium treatment in Trypanosoma spp. - infected dairy cattle in peri-urban Kampala, Uganda
Drug resistant trypanosome infections in livestock have been Reported from 13 sub-Saharan countries so far, in eight of which multiple resistance occurs. Since new trypanocides are unlikely to appear on the market in the near future, information about the occurrence, extent and impact of drug res...
Gall, Y.
,
Woitag, J.
,
Bauer, B.
,
Sidibe, I.
,
McDermott, John J.
,
Eisler, M.C.
,
Mehlitz, D.
,
Clausen, P.H.
,
[Detection of trypanocide failure in cattle: Diagnostic potential of PCR compared to parasitological methods]
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Detection of trypanocide failure in cattle: Diagnostic potential of PCR compared to parasitological methods
Mba, C
,
Fregene, Martin A.
,
Tohme, Joseph M.
,
[Towards a densely saturated molecular genetic map of cassava: applying the DarT in the development and genome location of polymorphic DNA fragments]
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Towards a densely saturated molecular genetic map of cassava: applying the DarT in the development and genome location of polymorphic DNA fragments
Diagnosis is an essential requirement in the management of disease both at the level of the individual, when a decision has to be made whether to treat or not, and at the epidemiological level for evaluating the performance of disease control strategies. The centrality of diagnosis in disease man...
Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...
Ouma, J.O.
,
Masake, R.A.
,
Masiga, Daniel K.
,
Moloo, S.K.
,
Minja, S.H.
,
Njuguna, J.T.
,
Olaho-Mukani, W.
,
Ndung'u, J.M.
,
[Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)]
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Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)
Andean potato mottle virus (APMoV, genero Comovirus) es un patogeno importante de varias plantas Solanaceas, que incluyen papa, tomate y tabaco, pero hasta la fecha solo esta disponible datos de secuencias parciales. En este estudio, se determino el genoma completo de APMoV utilizando secuenciami...
Souza, J. de
,
Muller, G.
,
Cuéllar, Wilmer J.
,
Kreuze, J.F.
,
[Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.]
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Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.
El virus colusivo del camote (SPCV, genero Cavemovirus), el virus del aclaramiento de venas del camote (SPVC, genero Solendovirus) y el virus del enrollamiento de hojas del camote (SPLCV, genero Begomovirus) son virus con genoma de ADN, presentes en el camote en infecciones virales simples o mult...
Berrocal, A.
,
Rossel, G.
,
Fuentes, S.
,
Pérez, A.
,
Cuéllar, Wilmer J.
,
Kreuze, J.F.
,
[Desarrollo y aplicacion de PCR multiple para la deteccion simultanea de tres virus de ADN en camote (Ipomoea batatas L.)]
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Desarrollo y aplicacion de PCR multiple para la deteccion simultanea de tres virus de ADN en camote (Ipomoea batatas L.)
The primary goals of applying molecular tools, developed at ILRI in conjunction with the use of infection and treatment immunisation are quality control of vaccines; monitoring of possible vaccine breakthrough and breakdown; and assessment of the impact of introducing new parasite types on long-t...
Bishop, Richard P.
,
Nene, Vishvanath
,
Spooner, P.R.
,
Mbogo, S.
,
Kariuki, D.
,
Payne, R.
,
Morzaria, S.P.
,
[Application of molecular tools in support of deployment of Theileria parva live vaccines]
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Application of molecular tools in support of deployment of Theileria parva live vaccines