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We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effecti...


Ballingall, K.T.Mwangi, D.M.MacHugh, Niall D.Taracha, E.L.N.Totte, P.McKeever, Declan J.[A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells]A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
Shapiro, S.Z.Young, J.R.[An immunochemical method for mRNA purification. Application to messenger RNA encoding trypanosome variable surface antigen]An immunochemical method for mRNA purification. Application to messenger RNA encoding trypanosome variable surface antigen
Gee, A.L.W. de[Host-parasite relationships in Trypanosoma (Duttonella ) vivax with special reference to the influence of antigenic variation]Host-parasite relationships in Trypanosoma (Duttonella ) vivax with special reference to the influence of antigenic variation

East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as f...


Kaba, S.A.Schaap, D.Roode, E.C.Nene, VishvanathMusoke, A.J.Vlak, J.M.Oers, M.M. van[Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens]Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens
Waithanji, Elizabeth M.Nantulya, E.M.Mbiuki, S.M.[Use of antigens capture tube enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma evansi infections in dromedary camels (Camelus dromedarius)]Use of antigens capture tube enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma evansi infections in dromedary camels (Camelus dromedarius)

Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card agglutination test (CATT) for the detection of Trypanosoma evansi infections in buffaloes in Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from 139 Indonesian buffaloes which ...


Davison, H.C.Thrusfield, M.V.Muharsini, S.Husein, A.Partoutomo, S.Rae, P.F.Masake, R.Luckins, A.G.[Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia]Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia
Baldwin, C.L.Teale, A.J.[Alloreactive T-cell clones transformed by Theileria parva retain cytolytic activity and antigen specificity]Alloreactive T-cell clones transformed by Theileria parva retain cytolytic activity and antigen specificity
Nantulya, V.M.Bajyana Songa, E.Hamers, R.[Detection of circulating trypanosomal antigen in Trypanosoma evansi-infected animals using a Trypanosoma brucei group-specific monoclonal antibody]Detection of circulating trypanosomal antigen in Trypanosoma evansi-infected animals using a Trypanosoma brucei group-specific monoclonal antibody
Wells, P.W.Emery, D.L.Hinson, C.A.Morrison, W. IvanMurray, M.[Immunization of cattle with variant-specific surface antigen of Trypanosoma brucei. The influence of different adjuvants]Immunization of cattle with variant-specific surface antigen of Trypanosoma brucei. The influence of different adjuvants

Theileria parva, a tick-transmitted protozoan parasite related to Plasmodium spp., causes the disease East Coast fever, an acute and usually fatal lymphoproliferative disorder of cattle in Africa. Previous studies using sera from cattle that have survived infection identified a polymorphic immuno...


Toye, Philip G.Metzelaar, M.J.Wijngaard, P.L.J.Nene, VishvanathIams, K.P.Roose, J.Nyanjui, J.K.Gobright, E.I.Musoke, A.J.Clevers, H.C.[Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva]Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva

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