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Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...


Otti, G.Bouvaine, S.Kimata, B.Mkamillo, G.Kumar, P.L.Tomlins, Keith I.Maruthi, M.N.[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies, as the disease is progressing westwards to new geographical areas. A stringen...


Anjanappa, R.B.Mehta, D.Maruthi, M.N.Kanju, E.Gruissem, W.Vanderschuren, H.[Characterization of brown streak virus–resistant cassava]Characterization of brown streak virus–resistant cassava

In a bid to increase the number of genotypes reaching farmers, on-farm trials were conducted in 2001/2002 with the aim of increasing the number of genotypes available to farmers to be evaluated for performance, adaptation and acceptability. Twenty improved genotypes were planted in Lira, Nakasong...


Ntawuruhunga, PheneasSsemakula, G.N.Ojulong, H.Bua, A.Ragama, P.E.Kanobe, C.Whyte, J.[Evaluation of advanced cassava genotypes in Uganda]Evaluation of advanced cassava genotypes in Uganda

Cassava anthracnose disease (CAD) caused by the fungus Colletotrichum gloeosporioides f.sp. manihotis has become one of the major economic diseases of cassava in Africa. The expansion of cassava production farmlands particularly in the humid rainfall zones of West and Central Africa has led to an...


Fokunang, C.N.Dixon, Alfred G.O.Ikotun, T.Tembe-Fokunang, E.A.Akem, C.N.Asiedu, Robert[Anthracnose: an economic disease of cassava in Africa]Anthracnose: an economic disease of cassava in Africa
Hernández, Luis MiguelSotelo, GuillermoBonilla, Ximena P.Álvarez, ElizabethMiles, John W.Worthington, Margaret[Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types]Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types
Goto,ShingoSasakura-Shimoda, FuyukoSuetsugu, MaiGómez Selvaraj, MichaelHayashi, NagaoYamazaki, MuneoIshitani, ManabuShimono, MasakiSugano, ShojiMatsushita, AkaneTanabata, TakanariTakatsuji, Hiroshi[Development of disease-resistant rice by optimized expression of WRKY45]Development of disease-resistant rice by optimized expression of WRKY45
Bentley, W.Martin, L.Hanna, R.[Impact of gray field ant exclusion from vines on grape mealybug abundance, parasitism, and infestation: a progress report]Impact of gray field ant exclusion from vines on grape mealybug abundance, parasitism, and infestation: a progress report

Cassava is the main crop in the Congo but its low yield doesn’t meet the needs of Congolese populations. The low yield is due to the use of less effective sensitive varieties to diseases, non-mastering of techniques and biotic constraint of which the African cassava mosaic. This study aims at sel...


Aimé, K.G.R.Mpika, J.Mahungu, M.M.Mikoko, M.E.Mabanza, J.Attibayeba[Evaluation of cassava hybrids performance obtained by controlled pollination of elite accessions from Niari landscape in the Republic of Congo]Evaluation of cassava hybrids performance obtained by controlled pollination of elite accessions from Niari landscape in the Republic of Congo

Tumwegamire, SilverKanju, E.Legg, J.P.Shirima, R.Kombo, S.Mkamilo, G.S.Mtunda, K.Sichalwe, K.Kulembeka, H.P.Ndyetabura, I.Saleh, H.Kawuki, R.Alicai, TitusAdiga, G.Benesi, I.Mhone, A.Zacarias, A.Matsimbe, S.F.Munga, T.Ateka, E.Navangi, L.Maruthi, M.N.Mwatuni, F.Ngundo, G.Mwangangi, M.Mbugua, E.Ndunguru, J.Rajabu, C.Mark, D.[Exchanging and managing in-vitro elite germplasm to combat cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) in Eastern and Southern Africa]Exchanging and managing in-vitro elite germplasm to combat cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) in Eastern and Southern Africa
Tuan Vu, NamPardo, Juan ManuelÁlvarez, ElizabethHuy Le, HamWyckhuys, Kris A.G.Nguyen, Kim-LienTien Le, Dung[Establishment of a loop-mediated isothermal amplification (LAMP) assay for the detection of phytoplasma-associated cassava witches’ broom disease]Establishment of a loop-mediated isothermal amplification (LAMP) assay for the detection of phytoplasma-associated cassava witches’ broom disease

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