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Journal Article 2012-01

Autor: Abarshi, M.M. Mohammed, I. Jeremiah, S.C. Legg, J.P. Kumar, P.L. Hillocks, R.J. Maruthi, M.N.

Uniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs con...

Enlace original: https://cgspace.cgiar.org/handle/10568/77415

Investigación en Agricultura
RNA
DNA
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Abarshi, M.M. ,  Mohammed, I. ,  Jeremiah, S.C. ,  Legg, J.P. ,  Kumar, P.L. ,  Hillocks, R.J. ,  Maruthi, M.N. ,  [Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa] ,  Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa
Journal Article 2016-05

Autor: Otti, G. Bouvaine, S. Kimata, B. Mkamillo, G. Kumar, P.L. Tomlins, Keith I. Maruthi, M.N.

Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...

Enlace original: https://cgspace.cgiar.org/handle/10568/76987

Investigación en Agricultura
DNA
Enlace permanente
Otti, G. ,  Bouvaine, S. ,  Kimata, B. ,  Mkamillo, G. ,  Kumar, P.L. ,  Tomlins, Keith I. ,  Maruthi, M.N. ,  [High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants] ,  High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants
Journal Article 2019

Autor: Tegally, A. Jaufeerally-Fakim, Y. Dulloo, M.E.
Enlace original: https://cgspace.cgiar.org/handle/10568/103735

Investigación en Agricultura
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Tegally, A. ,  Jaufeerally-Fakim, Y. ,  Dulloo, M.E. ,  [Molecular characterisation of Solanum melongena L. and the crop wild relatives, S. violaceum Ortega and S. torvum Sw., using phylogenetic/DNA barcoding markers] ,  Molecular characterisation of Solanum melongena L. and the crop wild relatives, S. violaceum Ortega and S. torvum Sw., using phylogenetic/DNA barcoding markers
Journal Article 2004

Autor: Herzberg, F. Mahungu, N.M. Mignouna, J. Kullaya, A.

Twenty four arbitrarily selected cassava (Manihot esculenta Crantz) landraces, supplied by the germplasm collections in Kibaha and Ukiriguru research stations of Tanzania, were analysed by two different molecular marker techniques. Genetic distances on the basis of RAPD (random amplified polymorp...

Enlace original: https://cgspace.cgiar.org/handle/10568/96410

Investigación en Agricultura
DNA
Enlace permanente

Herzberg, F. ,  Mahungu, N.M. ,  Mignouna, J. ,  Kullaya, A. ,  [Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers] ,  Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers
Journal Article 2017

Autor: Nie, X. Dickison, V.L. Brooks, S. Nie, B. Singh, M. Koeyer, D.L. de Murphy, A.M.

Assessment of the existing PCR-gel electrophoresis-based methods for detection of Rx1 and Rx2, the genes that independently control extreme resistance (ER) to Potato virus X (PVX), indicated that the 5Rx1F/5Rx1R primer pair led to reliable detection of Rx1 whereas the 106Rx2F/106Rx2R primer pair ...

Enlace original: https://cgspace.cgiar.org/handle/10568/89939

Investigación en Agricultura
DNA
Enlace permanente
Nie, X. ,  Dickison, V.L. ,  Brooks, S. ,  Nie, B. ,  Singh, M. ,  Koeyer, D.L. de ,  Murphy, A.M. ,  [High resolution DNA melting assays for detection of Rx1 and Rx2 for high-throughput marker assisted selection for extreme resistance to Potato virus X in tetraploid potato] ,  High resolution DNA melting assays for detection of Rx1 and Rx2 for high-throughput marker assisted selection for extreme resistance to Potato virus X in tetraploid potato
Journal Article 2018-06-27

Autor: Norman, P.E. Asfaw, A. Tongoona, P.B. Danquah, A. Danquah, E.Y. Koeyer, D. de Asiedu, Robert

Controlled pollination in root and tuber crops is challenging. Complex ploidy, cross-incompatibility, erratic flowering patterns, outcrossing, etc., limit the efficiency of breeding progress in these crops. Half-sib breeding that involves random pollination among parents is a viable method to har...

Enlace original: https://cgspace.cgiar.org/handle/10568/96137

Investigación en Agricultura
DNA
Enlace permanente

Norman, P.E. ,  Asfaw, A. ,  Tongoona, P.B. ,  Danquah, A. ,  Danquah, E.Y. ,  Koeyer, D. de ,  Asiedu, Robert ,  [Can parentage analysis facilitate breeding activities in root and tuber crops?] ,  Can parentage analysis facilitate breeding activities in root and tuber crops?
Journal Article 2016-08-31

Autor: Perea, Claudia Hoz, Juan Fernando de la Cruz, Daniel Felipe Lobaton, Juan David Izquierdo, Paulo Quintero, Juan Camilo Raatz, Bodo Duitama, Jorge

Background Therecent development and availability of different genotype by sequencing (GBS) protocols provided a cost-effective approach to perform high-resolution genomic analysis of entire populations in different species. The central component of all these protocols is the digestion of the ini...

Enlace original: https://cgspace.cgiar.org/handle/10568/76973

Investigación en Agricultura
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Perea, Claudia ,  Hoz, Juan Fernando de la ,  Cruz, Daniel Felipe ,  Lobaton, Juan David ,  Izquierdo, Paulo ,  Quintero, Juan Camilo ,  Raatz, Bodo ,  Duitama, Jorge ,  [Bioinformatic analysis of genotype by sequencing (GBS) data with NGSEP] ,  Bioinformatic analysis of genotype by sequencing (GBS) data with NGSEP
Journal Article 2018-10-04

Autor: Nemecková, A. Christelová, P. Cížková, J. Nyine, M. Houwe, I. van den Svacina, R. Uwimana, B. Swennen, R. Doležel, Jaroslav Hribova, E.

East African highland bananas (EAHBs) are staple food crop in Uganda, Tanzania, Burundi, and other countries in the African Great Lakes region. Even though several morphologically different types exist, all EAHBs are triploid and display minimal genetic variation. To provide more insights into th...

Enlace original: https://cgspace.cgiar.org/handle/10568/98284

Investigación en Agricultura
DNA
Enlace permanente

Nemecková, A. ,  Christelová, P. ,  Cížková, J. ,  Nyine, M. ,  Houwe, I. van den ,  Svacina, R. ,  Uwimana, B. ,  Swennen, R. ,  Doležel, Jaroslav ,  Hribova, E. ,  [Molecular and cytogenetic study of east African highland banana] ,  Molecular and cytogenetic study of east African highland banana
Journal Article 2017-07-21

Autor: Nzuki, I. Katari, M.S. Bredeson, J.V. Masumba, E. Kapinga, F. Salum, K. Mkamilo, G.S. Shah, Tushaar Lyons, J.B. Rokhsar, D.S. Rounsley, S. Myburg, A.A. Ferguson, Morag E.

Genetic mapping of quantitative trait loci (QTL) for resistance to cassava brown streak disease (CBSD), cassava mosaic disease (CMD), and cassava green mite (CGM) was performed using an F1 cross developed between the Tanzanian landrace, Kiroba, and a breeding line, AR37-80. The population was eva...

Enlace original: https://cgspace.cgiar.org/handle/10568/82970

Investigación en Agricultura
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Nzuki, I. ,  Katari, M.S. ,  Bredeson, J.V. ,  Masumba, E. ,  Kapinga, F. ,  Salum, K. ,  Mkamilo, G.S. ,  Shah, Tushaar ,  Lyons, J.B. ,  Rokhsar, D.S. ,  Rounsley, S. ,  Myburg, A.A. ,  Ferguson, Morag E. ,  [QTL mapping for pest and disease resistance in cassava and coincidence with some introgression regions derived from M. glaziovii] ,  QTL mapping for pest and disease resistance in cassava and coincidence with some introgression regions derived from M. glaziovii
Journal Article 2017-05-22

Autor: Abdulmalik, R.O. Menkir, A. Meseka, S.K. Unachukwu, N.N. Ado, S.G. Olarewaju, J.D. Aba, D.A. Hearne, S. Crossa, J. Gedil, M.

Marker-assisted recurrent selection (MARS) is a breeding method used to accumulate favorable alleles that for example confer tolerance to drought in inbred lines from several genomic regions within a single population. A bi-parental cross formed from two parents that combine resistance to Striga ...

Enlace original: https://cgspace.cgiar.org/handle/10568/82566

Investigación en Agricultura
Enlace permanente

Abdulmalik, R.O. ,  Menkir, A. ,  Meseka, S.K. ,  Unachukwu, N.N. ,  Ado, S.G. ,  Olarewaju, J.D. ,  Aba, D.A. ,  Hearne, S. ,  Crossa, J. ,  Gedil, M. ,  [Genetic gains in grain yield of a maize population improved through marker assisted recurrent selection under stress and non-stress conditions in west Africa] ,  Genetic gains in grain yield of a maize population improved through marker assisted recurrent selection under stress and non-stress conditions in west Africa

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