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Sefasi, A.Kreuze, J.F.Manrique, S.Kiggundu, A.Ssemakula, G.N.Mukasa, S.[Induction of somatic embryogenesis in recalcitrant sweetpotato (Ipomoea batatas L.) cultivars.]Induction of somatic embryogenesis in recalcitrant sweetpotato (Ipomoea batatas L.) cultivars.

A rapid and reproducible in vitro plant regeneration procedure was developed for embryonic axis explants of cowpea [Vigna unguiculata (L.) Walp]. Moderate levels of 6-benzylaminopurine (0.5-2 mg l-1) were effective in inducing multiple shoots on decapitated embryonic axes on a Murashige and Skoog...


Raji, A.A.Oriero, E.Odeseye, B.Odunlami, T.Ingelbrecht, I.L.[Plant regeneration and Agrobacteriummediated transformation of African cowpea (Vigna unguiculata (L.) Walp) genotypes using embryonic axis explants]Plant regeneration and Agrobacteriummediated transformation of African cowpea (Vigna unguiculata (L.) Walp) genotypes using embryonic axis explants

Shoot regeneration via organogenesis was achieved from axenic cowpea [Vigna unguiculata subsp. unguiculata L. (Walp.) Verde.] hypocotyls and cotyledons of advanced breeding lines and varieties. Cotyledons and embryos were excised from green immature pods. The apical parts of the embryos were remo...


Pellegrineschi, A.[In vitro plant regeneration via organogenesis of cowpea [ Vigna unguiculata (l.) Walp]]In vitro plant regeneration via organogenesis of cowpea [ Vigna unguiculata (l.) Walp]

This 14-minute video describes the protocol used for the collection of female flowers at different ages after anthesis and the development of a protocol of early embryo rescue. The production of doubled haploids through different gynogenesis approaches usually require an early rescue of embryos w...


Buitrago, María EugeniaTabares, EddieLentini, ZaidaVanegas, FreddyWedzony, MariaCeballos, Hernán[Protocol of ovule culture for rescuing embryos in cassava = Protocolo para el cultivo de óvulos en el rescate de embriones en yuca/mandioca]Protocol of ovule culture for rescuing embryos in cassava = Protocolo para el cultivo de óvulos en el rescate de embriones en yuca/mandioca

This 9-minute video describes the protocol used for the collection of male flowers in cassava for the production of doubled haploids through androgenesis (in vitro microspore or anther culture). Pre-treatments and different culture conditions used in the process to induce cell division in immatur...


Perera, Prasanthi I.P.Golemiec, ElzbietaDedicova, BeataWedzony, MariaHeberle-Bors, ErwinOrdoñez, CarlosCeballos, Hernán[Protocol of anther and microspore culture in cassava (androgenesis) = Protocolo para el cultivo de anteras y microsporas (androgénesis) en yuca/mandioca]Protocol of anther and microspore culture in cassava (androgenesis) = Protocolo para el cultivo de anteras y microsporas (androgénesis) en yuca/mandioca

Over the last three decades, sporadic efforts have been made to develop regeneration and transformation systems in cowpea ( Vigna unguiculata L. Walp). This paper reviews the progress made to date, including highlights of culture media and explants used for regeneration and chimeric gene construc...


Machuka, J.Adesoye, A.Obembe, O.O.[Regeneration and genetic transformation in cowpea]Regeneration and genetic transformation in cowpea
Segovia, VSánchez, I.Mejía Jiménez, ARoca, William M.Lentini, Z[Micropropagación y regeneración de lulo (Solanum quitoense) por organogénesis]Micropropagación y regeneración de lulo (Solanum quitoense) por organogénesis
Litz, REJarret, Robert L.[Regeneración de plantas en el cultivo de tejidos: embriogénesis somática y organogénesis]Regeneración de plantas en el cultivo de tejidos: embriogénesis somática y organogénesis

Genotypic differences in the ability of immature leaf lobes and apical shoot meristems of cassava to form primary somatic embryos in P-CIM were observed (p ≤ 0.05). The mean number of apical meristems forming primary organized embryogenic structures when cultured in embryo induction medium supple...


Hankoua, B.Ng, S.Fawole, I.Puonti-Kaerlas, J.Pillay, M.Dixon, A.[Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the fieldestablished regenerants]Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the fieldestablished regenerants
Martínez Friz, Paolo Marcial[Organogénesis indirecta a partir de embriones maduros de Pinus radiata D. Don]Organogénesis indirecta a partir de embriones maduros de Pinus radiata D. Don

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