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A rapid and reproducible in vitro plant regeneration procedure was developed for embryonic axis explants of cowpea [Vigna unguiculata (L.) Walp]. Moderate levels of 6-benzylaminopurine (0.5-2 mg l-1) were effective in inducing multiple shoots on decapitated embryonic axes on a Murashige and Skoog...
Shoot regeneration via organogenesis was achieved from axenic cowpea [Vigna unguiculata subsp. unguiculata L. (Walp.) Verde.] hypocotyls and cotyledons of advanced breeding lines and varieties. Cotyledons and embryos were excised from green immature pods. The apical parts of the embryos were remo...
This 14-minute video describes the protocol used for the collection of female flowers at different ages after anthesis and the development of a protocol of early embryo rescue. The production of doubled haploids through different gynogenesis approaches usually require an early rescue of embryos w...
This 9-minute video describes the protocol used for the collection of male flowers in cassava for the production of doubled haploids through androgenesis (in vitro microspore or anther culture). Pre-treatments and different culture conditions used in the process to induce cell division in immatur...
Over the last three decades, sporadic efforts have been made to develop regeneration and transformation systems in cowpea ( Vigna unguiculata L. Walp). This paper reviews the progress made to date, including highlights of culture media and explants used for regeneration and chimeric gene construc...
Genotypic differences in the ability of immature leaf lobes and apical shoot meristems of cassava to form primary somatic embryos in P-CIM were observed (p ≤ 0.05). The mean number of apical meristems forming primary organized embryogenic structures when cultured in embryo induction medium supple...
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